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LPixel Inc lpx imagej plugin
Quantification of wound assay, Golgi appearance and microtubule distribution in patient-derived fibroblasts compared to control . (a) Representative images of sub-confluent fibroblast cells derived from either the index individual in Family 1 (IV:1) or control cell lines at 0 h of wound-induced cell migration assay. Scale bar: 1000 μm. (b) Wound closure was followed serially for 29 h during which a significant difference in cell migration was observed between the two cell lines. Scale bar: 1000 μm. (c) Percent wound closure was evaluated up to 29 h. (d) The remaining area of the wound was measured in μm 2 . The percent wound closure and area of the wound were measured using the <t>ImageJ</t> <t>plugin</t> Wound healing size tool image tool analysis ( https://github.com/AlejandraArnedo/Wound-healing-size-tool/wiki ). Data from 50 cells were collected and analysed. (e) Control cells showing perinuclear compact and polarized Golgi apparatus that are directed toward the migrating wound edge. (f) IV:1-derived fibroblast cells depicting a dispersed and non-polarized (oriented) Golgi apparatus towards the migrating/wound edge. (g) A representative image of normally polarized and parallel orientated microtubules in control cells. (h) A representative image of individual IV:1-derived fibroblast cells showing an un-polarized chaotic microtubule distribution; microtubules labelled with α-tubulin (green) and the centrosome is labelled with Pericentrin (orange) antibodies. (i) The total area of Golgi from 100 cells was measured using the ImageJ software. Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the <t>LPX</t> ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells. Data from 50 cells were collected and analysed and p-value was calculated based on Welch's t-test for (c, d, i, j and k). The red squares represent datapoints collected from control sample, the green circles represent datapoints collected from the patient sample (IV:1). The horizontal line represents the mean.
Lpx Imagej Plugin, supplied by LPixel Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imagej+plugin/imagej+plugins/pmc12139437-290-41-45
Average 90 stars, based on 1 article reviews
lpx imagej plugin - by Bioz Stars, 2026-09
90/100 stars

Images

1) Product Images from "SLK is mutated in individuals with a neurodevelopmental disorder"

Article Title: SLK is mutated in individuals with a neurodevelopmental disorder

Journal: eBioMedicine

doi: 10.1016/j.ebiom.2025.105725

Quantification of wound assay, Golgi appearance and microtubule distribution in patient-derived fibroblasts compared to control . (a) Representative images of sub-confluent fibroblast cells derived from either the index individual in Family 1 (IV:1) or control cell lines at 0 h of wound-induced cell migration assay. Scale bar: 1000 μm. (b) Wound closure was followed serially for 29 h during which a significant difference in cell migration was observed between the two cell lines. Scale bar: 1000 μm. (c) Percent wound closure was evaluated up to 29 h. (d) The remaining area of the wound was measured in μm 2 . The percent wound closure and area of the wound were measured using the ImageJ plugin Wound healing size tool image tool analysis ( https://github.com/AlejandraArnedo/Wound-healing-size-tool/wiki ). Data from 50 cells were collected and analysed. (e) Control cells showing perinuclear compact and polarized Golgi apparatus that are directed toward the migrating wound edge. (f) IV:1-derived fibroblast cells depicting a dispersed and non-polarized (oriented) Golgi apparatus towards the migrating/wound edge. (g) A representative image of normally polarized and parallel orientated microtubules in control cells. (h) A representative image of individual IV:1-derived fibroblast cells showing an un-polarized chaotic microtubule distribution; microtubules labelled with α-tubulin (green) and the centrosome is labelled with Pericentrin (orange) antibodies. (i) The total area of Golgi from 100 cells was measured using the ImageJ software. Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the LPX ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells. Data from 50 cells were collected and analysed and p-value was calculated based on Welch's t-test for (c, d, i, j and k). The red squares represent datapoints collected from control sample, the green circles represent datapoints collected from the patient sample (IV:1). The horizontal line represents the mean.
Figure Legend Snippet: Quantification of wound assay, Golgi appearance and microtubule distribution in patient-derived fibroblasts compared to control . (a) Representative images of sub-confluent fibroblast cells derived from either the index individual in Family 1 (IV:1) or control cell lines at 0 h of wound-induced cell migration assay. Scale bar: 1000 μm. (b) Wound closure was followed serially for 29 h during which a significant difference in cell migration was observed between the two cell lines. Scale bar: 1000 μm. (c) Percent wound closure was evaluated up to 29 h. (d) The remaining area of the wound was measured in μm 2 . The percent wound closure and area of the wound were measured using the ImageJ plugin Wound healing size tool image tool analysis ( https://github.com/AlejandraArnedo/Wound-healing-size-tool/wiki ). Data from 50 cells were collected and analysed. (e) Control cells showing perinuclear compact and polarized Golgi apparatus that are directed toward the migrating wound edge. (f) IV:1-derived fibroblast cells depicting a dispersed and non-polarized (oriented) Golgi apparatus towards the migrating/wound edge. (g) A representative image of normally polarized and parallel orientated microtubules in control cells. (h) A representative image of individual IV:1-derived fibroblast cells showing an un-polarized chaotic microtubule distribution; microtubules labelled with α-tubulin (green) and the centrosome is labelled with Pericentrin (orange) antibodies. (i) The total area of Golgi from 100 cells was measured using the ImageJ software. Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the LPX ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells. Data from 50 cells were collected and analysed and p-value was calculated based on Welch's t-test for (c, d, i, j and k). The red squares represent datapoints collected from control sample, the green circles represent datapoints collected from the patient sample (IV:1). The horizontal line represents the mean.

Techniques Used: Derivative Assay, Control, Cell Migration Assay, Migration, Software

Related Articles

other:

Article Title: SLK is mutated in individuals with a neurodevelopmental disorder
Article Snippet: Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the LPX ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells.

Article Title: Plant chromosome polytenization contributes to suppression of root growth in high polyploids.
Article Snippet: Autopolyploidization, which refers to a polyploidization via genome duplication without hybridization, promotes growth in autotetraploids, but suppresses growth in high polyploids (autohexaploids or auto-octoploids).. The mechanism underlying this growth suppression (i.e. ‘high-ploidy syndrome’) has not been comprehensively characterized.. In this study, we conducted a kinematic analysis of the root apical meristem cells in Arabidopsis thaliana autopolyploids (diploid, tetraploid, hexaploid, and octoploid) to determine the effects of the progression of genome duplication on root growth.

Article Title: Evaluation of motion artifacts reduction software that compensate for respiratory movements in the craniocaudal direction during abdominal cone-beam computed tomography.
Article Snippet: We acquired cone-beam computed tomography (CBCT) images of a locally made contrast-enhanced hepatic artery phantom under various conditions, both with the phantom still, and while moving it from the cranial to the caudal position.. All the motion CBCT images were processed with and without motion artifacts reduction software (MARS).. We calculated some quantitative similarity indexes between the still CBCT images (no-motion) and the motion CBCT images both processed with MARS (MARS ON) and without MARS (MARS OFF).

Generated:

Article Title: A reversible metabolic stress-sensitive regulation of CRMP2A orchestrates EMT/stemness and increases metastatic potential in cancer.
Article Snippet: .. Bottom images in each group are high magnifications of representative areas (yellow boxes) from the images above. (C) Microtubule skeleton images generated via an ImageJ plugin (LPIXEL) based on tubulin staining and confocal imaging photomicrographs were used to quantify the branching points of microtubules, showing increased branching in CRMP2A-deficient cells (means and SEM are shown, n = 6 fields/group/plate, *p < 0.05 compared with control cells for baseline; #p < 0.05 compared with control for low glucose). ..

Staining:

Article Title: A reversible metabolic stress-sensitive regulation of CRMP2A orchestrates EMT/stemness and increases metastatic potential in cancer.
Article Snippet: .. Bottom images in each group are high magnifications of representative areas (yellow boxes) from the images above. (C) Microtubule skeleton images generated via an ImageJ plugin (LPIXEL) based on tubulin staining and confocal imaging photomicrographs were used to quantify the branching points of microtubules, showing increased branching in CRMP2A-deficient cells (means and SEM are shown, n = 6 fields/group/plate, *p < 0.05 compared with control cells for baseline; #p < 0.05 compared with control for low glucose). ..

Imaging:

Article Title: A reversible metabolic stress-sensitive regulation of CRMP2A orchestrates EMT/stemness and increases metastatic potential in cancer.
Article Snippet: .. Bottom images in each group are high magnifications of representative areas (yellow boxes) from the images above. (C) Microtubule skeleton images generated via an ImageJ plugin (LPIXEL) based on tubulin staining and confocal imaging photomicrographs were used to quantify the branching points of microtubules, showing increased branching in CRMP2A-deficient cells (means and SEM are shown, n = 6 fields/group/plate, *p < 0.05 compared with control cells for baseline; #p < 0.05 compared with control for low glucose). ..

Control:

Article Title: A reversible metabolic stress-sensitive regulation of CRMP2A orchestrates EMT/stemness and increases metastatic potential in cancer.
Article Snippet: .. Bottom images in each group are high magnifications of representative areas (yellow boxes) from the images above. (C) Microtubule skeleton images generated via an ImageJ plugin (LPIXEL) based on tubulin staining and confocal imaging photomicrographs were used to quantify the branching points of microtubules, showing increased branching in CRMP2A-deficient cells (means and SEM are shown, n = 6 fields/group/plate, *p < 0.05 compared with control cells for baseline; #p < 0.05 compared with control for low glucose). ..



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Quantification of wound assay, Golgi appearance and microtubule distribution in patient-derived fibroblasts compared to control . (a) Representative images of sub-confluent fibroblast cells derived from either the index individual in Family 1 (IV:1) or control cell lines at 0 h of wound-induced cell migration assay. Scale bar: 1000 μm. (b) Wound closure was followed serially for 29 h during which a significant difference in cell migration was observed between the two cell lines. Scale bar: 1000 μm. (c) Percent wound closure was evaluated up to 29 h. (d) The remaining area of the wound was measured in μm 2 . The percent wound closure and area of the wound were measured using the <t>ImageJ</t> <t>plugin</t> Wound healing size tool image tool analysis ( https://github.com/AlejandraArnedo/Wound-healing-size-tool/wiki ). Data from 50 cells were collected and analysed. (e) Control cells showing perinuclear compact and polarized Golgi apparatus that are directed toward the migrating wound edge. (f) IV:1-derived fibroblast cells depicting a dispersed and non-polarized (oriented) Golgi apparatus towards the migrating/wound edge. (g) A representative image of normally polarized and parallel orientated microtubules in control cells. (h) A representative image of individual IV:1-derived fibroblast cells showing an un-polarized chaotic microtubule distribution; microtubules labelled with α-tubulin (green) and the centrosome is labelled with Pericentrin (orange) antibodies. (i) The total area of Golgi from 100 cells was measured using the ImageJ software. Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the <t>LPX</t> ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells. Data from 50 cells were collected and analysed and p-value was calculated based on Welch's t-test for (c, d, i, j and k). The red squares represent datapoints collected from control sample, the green circles represent datapoints collected from the patient sample (IV:1). The horizontal line represents the mean.
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Image Search Results


Quantification of wound assay, Golgi appearance and microtubule distribution in patient-derived fibroblasts compared to control . (a) Representative images of sub-confluent fibroblast cells derived from either the index individual in Family 1 (IV:1) or control cell lines at 0 h of wound-induced cell migration assay. Scale bar: 1000 μm. (b) Wound closure was followed serially for 29 h during which a significant difference in cell migration was observed between the two cell lines. Scale bar: 1000 μm. (c) Percent wound closure was evaluated up to 29 h. (d) The remaining area of the wound was measured in μm 2 . The percent wound closure and area of the wound were measured using the ImageJ plugin Wound healing size tool image tool analysis ( https://github.com/AlejandraArnedo/Wound-healing-size-tool/wiki ). Data from 50 cells were collected and analysed. (e) Control cells showing perinuclear compact and polarized Golgi apparatus that are directed toward the migrating wound edge. (f) IV:1-derived fibroblast cells depicting a dispersed and non-polarized (oriented) Golgi apparatus towards the migrating/wound edge. (g) A representative image of normally polarized and parallel orientated microtubules in control cells. (h) A representative image of individual IV:1-derived fibroblast cells showing an un-polarized chaotic microtubule distribution; microtubules labelled with α-tubulin (green) and the centrosome is labelled with Pericentrin (orange) antibodies. (i) The total area of Golgi from 100 cells was measured using the ImageJ software. Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the LPX ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells. Data from 50 cells were collected and analysed and p-value was calculated based on Welch's t-test for (c, d, i, j and k). The red squares represent datapoints collected from control sample, the green circles represent datapoints collected from the patient sample (IV:1). The horizontal line represents the mean.

Journal: eBioMedicine

Article Title: SLK is mutated in individuals with a neurodevelopmental disorder

doi: 10.1016/j.ebiom.2025.105725

Figure Lengend Snippet: Quantification of wound assay, Golgi appearance and microtubule distribution in patient-derived fibroblasts compared to control . (a) Representative images of sub-confluent fibroblast cells derived from either the index individual in Family 1 (IV:1) or control cell lines at 0 h of wound-induced cell migration assay. Scale bar: 1000 μm. (b) Wound closure was followed serially for 29 h during which a significant difference in cell migration was observed between the two cell lines. Scale bar: 1000 μm. (c) Percent wound closure was evaluated up to 29 h. (d) The remaining area of the wound was measured in μm 2 . The percent wound closure and area of the wound were measured using the ImageJ plugin Wound healing size tool image tool analysis ( https://github.com/AlejandraArnedo/Wound-healing-size-tool/wiki ). Data from 50 cells were collected and analysed. (e) Control cells showing perinuclear compact and polarized Golgi apparatus that are directed toward the migrating wound edge. (f) IV:1-derived fibroblast cells depicting a dispersed and non-polarized (oriented) Golgi apparatus towards the migrating/wound edge. (g) A representative image of normally polarized and parallel orientated microtubules in control cells. (h) A representative image of individual IV:1-derived fibroblast cells showing an un-polarized chaotic microtubule distribution; microtubules labelled with α-tubulin (green) and the centrosome is labelled with Pericentrin (orange) antibodies. (i) The total area of Golgi from 100 cells was measured using the ImageJ software. Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the LPX ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells. Data from 50 cells were collected and analysed and p-value was calculated based on Welch's t-test for (c, d, i, j and k). The red squares represent datapoints collected from control sample, the green circles represent datapoints collected from the patient sample (IV:1). The horizontal line represents the mean.

Article Snippet: Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the LPX ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells.

Techniques: Derivative Assay, Control, Cell Migration Assay, Migration, Software